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Mini-Circuits
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GE Healthcare
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Proteintech
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Image Search Results
Journal: Frontiers in Medicine
Article Title: IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization
doi: 10.3389/fmed.2022.754341
Figure Lengend Snippet: Primers sequences of target gene.
Article Snippet: After blocking with 5% BSA (Meilunbio, Dalian, China) for 1 h, the membrane was incubated with primary antibodies of GAPDH (1:2,000, Proteintech, Wuhan, China), TGF-β1 (1:1,000, ABclonal, Wuhan, China), IL-6 (1:2,000, Proteintech, Wuhan, China), PD-L1 (1:2,000, Proteintech, Wuhan, China),
Techniques:
Journal: Frontiers in Medicine
Article Title: IL-7-Treated Periodontal Ligament Cells Regulate Local Immune Homeostasis by Modulating Treg/Th17 Cell Polarization
doi: 10.3389/fmed.2022.754341
Figure Lengend Snippet: Effects of treatment with different concentrations of rhIL-7 in hPDLCs on Notch1 signaling pathway-related molecules. (A–C) After hPDLCs were treated with different concentrations of IL-7 for 24 h, the mRNA level and protein level of Notch1, Jagged1, and Hes1 were detected by RT-qPCR and WB. n = 3, * p < 0.05. ** p < 0.01. ## DAPT group compared with 200 ng/mL rhIL-7 group, p < 0.01. hPDLCs, human periodontal ligament cells; RT-qPCR, reverse transcription quantitative PCR; WB, Western blot.
Article Snippet: After blocking with 5% BSA (Meilunbio, Dalian, China) for 1 h, the membrane was incubated with primary antibodies of GAPDH (1:2,000, Proteintech, Wuhan, China), TGF-β1 (1:1,000, ABclonal, Wuhan, China), IL-6 (1:2,000, Proteintech, Wuhan, China), PD-L1 (1:2,000, Proteintech, Wuhan, China),
Techniques: Quantitative RT-PCR, Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot